Food Allergens: Methods and Protocols (Methods in Molecular Biology) 1st Edition 2017
This volume provides a collection of methodologies for basic research clinical diagnosis and treatment pertaining to food allergens including food allergen production purification characterization detection quantification and bioinformatics approaches to modern food allergen studies. The chapters in the book are divided into 4 parts: Part I discusses food allergen purification and production and explores methods of producing recombinant food allergens in bacterial and yeast expression systems; Part II looks at allergen discovery detection and quantification covering 3 types of methods?DNA- protein- and cell-based methods; Part III focuses on allergenic epitope mapping; and Part IV talks about future developments concentrated around new concepts of allergenicity as an outcome of protein and food matrix interactions. Written in the highly successful Methods in Molecular Biology series format chapters include introductions to their respective topics lists of the necessary materials and reagents step-by-step readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive Food Allergens: Methods and Protocols is a valuable resource for immunologists biochemists molecular biologists and medical doctors and students working in the food allergy field. This book is also useful for people in the food industry legislators food standard agencies allergologists pediatricians and clinicians in the allergic diseases and immunology fields.